Cell Localization of DPI-Dependent Production of Superoxide in Reproductive Tissues of the Olive Tree (Olea europaea L.)

Reactive Oxygen Species (ROS) are compounds derived from oxygen with important implications in biological processes plants, some of them related to reproduction. Among ROS, superoxide is the primary oxidant, since an array other ROS eventually this anion. Therefore, analysis molecular systems able generate molecule and cellular compartmentalization these events paramount importance. We have used fluorochrome DCFH2-DA chromogenic substrate NBT association DPI (a specific inhibitor Rboh enzymes generating plants) combination confocal microscopy stereomicroscopy, respectively identify cell localization general, accumulation olive reproductive tissues. A significant production both has been described, showing a fairly precise spatial temporal location throughout flower development. The reduction signal after addition suggests that generation largely due or flavin oxidase activity. At subcellular level, O2●− located plasma membrane mature pollen germinated pollen, as well rough endoplasmic reticulum mitochondria.